Synthetic Gene Recruitment Reveals Adaptive Reprogramming of Gene Regulation in Yeast

Synthetic gene recruitment reveals adaptive reprogramming of gene regulation in yeast.

The recruitment of a gene to a foreign regulatory system is a major evolutionary event that can lead to novel phenotypes. However, the evolvability potential of cells depends on their ability to cope with challenges presented by gene recruitment. To study this ability, we combined synthetic gene recruitment with continuous culture and online measurements of the metabolic and regulatory dynamics...

Correlated Noise in a Synthetic Gene Circuit Reveals Regulation

Simulations and analysis in the previous chapter are based on parameters for the λ cI system. Here, we test those predictions experimentally by building a synthetic circuit using the λ cI repressor and other characterized circuit elements in E. coli bacteria. The synthetic circuit uses three fluorescent proteins so that noisy gene expression can be monitored with time-lapse microscopy. To apply...

the study of aaag repeat polymorphism in promoter of errg gene and its association with the risk of breast cancer in isfahan region

چکیده: سرطان پستان دومین عامل مرگ مرتبط با سرطان در خانم ها است. از آنجا که سرطان پستان یک تومور وابسته به هورمون است، می تواند توسط وضعیت هورمون های استروئیدی شامل استروژن و پروژسترون تنظیم شود. استروژن نقش مهمی در توسعه و پیشرفت سرطان پستان ایفا می کند و تاثیر خود را روی بیان ژن های هدف از طریق گیرنده های استروژن اعمال می کند. اما گروه دیگری از گیرنده های هسته ای به نام گیرنده های مرتبط به ا...

Selection of tetracycline inducible self-cleaving ribozymes as synthetic devices for gene regulation in yeast.

Synthetic regulatory devices are key components for the development of complex biological systems and the reprogramming of cellular functions and networks. Here we describe the selection of tetracycline inducible hammerhead ribozymes. A tetracycline aptamer was fused to the full-length hammerhead ribozyme via a variable linker region. 11 rounds of in vitro selection were applied to isolate link...

Synthetic Hypermutation in Yeast Segovia et al . 1 Synthetic hypermutation : gene - drug mutation rate synergy reveals a translesion synthesis mechanism